![]() Bacterial culture: 24-48 hours bacterial culture. ![]() In this test urea broth is use which shows formation of pink color at alkaline pH. This change in pH can be use as identification parameter for bacteria by using pH sensitive media. Due to formation of ammonia, pH becomes alkaline. Some bacteria have ability to produced enzyme urease which breakdown the urea into ammonia and carbon dioxide. It excreted in urine as a nitrogenous waste product. Urea is water soluble, nitrogenous compound which is produces in urea cycle by decarboxylation of amino acid arginine. Urease Detection TestĪim: To check the activity of bacteria to degrade urea by the enzyme urease. Result: A clear zone observed around the bacterial colony of Bacillus cereus indicates the degradation of CMC by the presence of extracellular enzyme cellulase. Observation: Examine the plates for the formation of a clear zone around the colony. Flood the plates with 1% (v/v) aqueous solution of hexadecyltrimethylammonium bromide and observe.Keep inoculated plates in an inverted position at 35☌ for 2-4 days.Inoculate the respective organism in the plates.Pour the media into Petri dishes under aseptic condition and labeled them with the name of the microorganism to be inoculated.Prepared the Czapek mineral salt agar medium and sterilize in an autoclave as per standard.Chemicals: Hexadecyltrimethyl ammonium bromide.Media: Czapek mineral salt medium (pH 7.3 + 0.3).Apparatus: Glass rod, Petri dishes, glass marking pencil, inoculating loop.Bacterial culture: Nutrient agar plate culture for bacterial species like Bacillus cereus, Potato dextrose agar plate for fungal species like Aspergillus species.If bacteria cause degradation of cellulose it forms clear zone around bacterial colonies. ![]() It can be used for identification of cellulase producing bacteria like Acetobacter, Rhizobium, Achromobacter, Escherichia and Sarcina.Įvidence for microbial utilization of cellulose is detected by adding hexadecyltrimethyl ammonium bromide added in media. Degradation of cellulose is brought by some bacterial species with the help of the enzyme cellulase. Chemically it is polysaccharide of glucose residues with beta 1, 4 glycosidic linkage. Observe the plates for starch hydrolysis around the streaking of each organism and check the color change of the medium,ĭiscoloration of iodine around colonies indicates hydrolysis of starch by amylase and vice-Īim: To perform cellulase production testĬellulose is a basic structural material of plant species.
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